Immunocytochemistry:Article Title: Table S2 - Antibody Sources and Conditions for Immunocytochemistry
Article Snippet: .. Table S2 - Antibody Sources and Conditions for Immunocytochemistry Gene Name Host Species Supplier / Catalogue number Staining Method Dilution Antigen Retrieval CXCR4 Mouse IgG2A - PE R&D Systems FAB170P Fixed cell Flow 1:50 None SOX17 Goat IgG APC R&D Systems IC1924A Fixed cell Flow 1:50 None OCT4 Goat R&D Systems AF1759 4% PFA fixed monolayer 1:500 None PDX1 Guinea Pig Abcam Ab47308 4% PFA fixed monolayer 1:250 None pRb S780 Rabbit Cell Signalling 9307 4% PFA fixed monolayer 1:350 None PDX1 Guinea Pig Abcam Ab47308 PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER PDX1 Rabbit Dr. J. Habener Gift PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER Insulin Guinea Pig Sigma I8510 PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER Glucagon Rabbit Thermo Scientific PA1-37768 PFA fixed, paraffin section of agarose embedded cell pellet 1:200 HIER Glucagon Mouse Sigma G2654 PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER Somatostatin Mouse BCBC AB1985 PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER Pancreatic Polypeptide Goat R&D Systems AF6297 PFA fixed, paraffin section of agarose embedded cell pellet 1:200 HIER NKX6.1 Rabbit Dr. A. Rezania Gift PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER ARX Rabbit Dr. P. Collombat Gift PFA fixed, paraffin section of agarose embedded cell pellet 1:500 HIER PAX6 Rabbit Covance PRB-278P PFA fixed, paraffin section of agarose embedded cell pellet 1:250 HIER HIER (heat induced epitope retrieval): 15 minutes at 95°C in 10mM Citrate buffer with 0.05% Tween-20 pH 6.0. .. BCBC (Beta Cell Biology Consortium) Affiliations of Antibody Gifts: Dr. Habener: Laboratory of Molecular Endocrinology; Massachusetts General Hospital; Boston, MA USA Dr. Rezania: BetaLogics Venture, Janssen R&D LLC, Raritan, New Jersey, USA.
Staining:Article Title: Table S2 - Antibody Sources and Conditions for Immunocytochemistry
Article Snippet: .. Table S2 - Antibody Sources and Conditions for Immunocytochemistry Gene Name Host Species Supplier / Catalogue number Staining Method Dilution Antigen Retrieval CXCR4 Mouse IgG2A - PE R&D Systems FAB170P Fixed cell Flow 1:50 None SOX17 Goat IgG APC R&D Systems IC1924A Fixed cell Flow 1:50 None OCT4 Goat R&D Systems AF1759 4% PFA fixed monolayer 1:500 None PDX1 Guinea Pig Abcam Ab47308 4% PFA fixed monolayer 1:250 None pRb S780 Rabbit Cell Signalling 9307 4% PFA fixed monolayer 1:350 None PDX1 Guinea Pig Abcam Ab47308 PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER PDX1 Rabbit Dr. J. Habener Gift PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER Insulin Guinea Pig Sigma I8510 PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER Glucagon Rabbit Thermo Scientific PA1-37768 PFA fixed, paraffin section of agarose embedded cell pellet 1:200 HIER Glucagon Mouse Sigma G2654 PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER Somatostatin Mouse BCBC AB1985 PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER Pancreatic Polypeptide Goat R&D Systems AF6297 PFA fixed, paraffin section of agarose embedded cell pellet 1:200 HIER NKX6.1 Rabbit Dr. A. Rezania Gift PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER ARX Rabbit Dr. P. Collombat Gift PFA fixed, paraffin section of agarose embedded cell pellet 1:500 HIER PAX6 Rabbit Covance PRB-278P PFA fixed, paraffin section of agarose embedded cell pellet 1:250 HIER HIER (heat induced epitope retrieval): 15 minutes at 95°C in 10mM Citrate buffer with 0.05% Tween-20 pH 6.0. .. BCBC (Beta Cell Biology Consortium) Affiliations of Antibody Gifts: Dr. Habener: Laboratory of Molecular Endocrinology; Massachusetts General Hospital; Boston, MA USA Dr. Rezania: BetaLogics Venture, Janssen R&D LLC, Raritan, New Jersey, USA.
Paraffin Section:Article Title: Table S2 - Antibody Sources and Conditions for Immunocytochemistry
Article Snippet: .. Table S2 - Antibody Sources and Conditions for Immunocytochemistry Gene Name Host Species Supplier / Catalogue number Staining Method Dilution Antigen Retrieval CXCR4 Mouse IgG2A - PE R&D Systems FAB170P Fixed cell Flow 1:50 None SOX17 Goat IgG APC R&D Systems IC1924A Fixed cell Flow 1:50 None OCT4 Goat R&D Systems AF1759 4% PFA fixed monolayer 1:500 None PDX1 Guinea Pig Abcam Ab47308 4% PFA fixed monolayer 1:250 None pRb S780 Rabbit Cell Signalling 9307 4% PFA fixed monolayer 1:350 None PDX1 Guinea Pig Abcam Ab47308 PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER PDX1 Rabbit Dr. J. Habener Gift PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER Insulin Guinea Pig Sigma I8510 PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER Glucagon Rabbit Thermo Scientific PA1-37768 PFA fixed, paraffin section of agarose embedded cell pellet 1:200 HIER Glucagon Mouse Sigma G2654 PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER Somatostatin Mouse BCBC AB1985 PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER Pancreatic Polypeptide Goat R&D Systems AF6297 PFA fixed, paraffin section of agarose embedded cell pellet 1:200 HIER NKX6.1 Rabbit Dr. A. Rezania Gift PFA fixed, paraffin section of agarose embedded cell pellet 1:1000 HIER ARX Rabbit Dr. P. Collombat Gift PFA fixed, paraffin section of agarose embedded cell pellet 1:500 HIER PAX6 Rabbit Covance PRB-278P PFA fixed, paraffin section of agarose embedded cell pellet 1:250 HIER HIER (heat induced epitope retrieval): 15 minutes at 95°C in 10mM Citrate buffer with 0.05% Tween-20 pH 6.0. .. BCBC (Beta Cell Biology Consortium) Affiliations of Antibody Gifts: Dr. Habener: Laboratory of Molecular Endocrinology; Massachusetts General Hospital; Boston, MA USA Dr. Rezania: BetaLogics Venture, Janssen R&D LLC, Raritan, New Jersey, USA.
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